Isolation of thymidine diphosphate D-glucose, thymidine diphosphate D-galactose, and thymidine diphosphate 4-acetamido-4, 6-dideoxy-D-galactose from Pasteurella pseudotuberculosis

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Soluble uridine diphosphate D-galactose: D-glucose beta-4-D-galactosyltransferase from bovine milk.

Some of the properties of a 70-fold purified bovine milk lactose synthetase have been determined. The enzyme preparation appears to be specific for uracil nucleoside diphosphate D-galactose derivatives. Adenosine, thymidine, @dine, or guanosine diphosphate derivatives cannot serve as galactosyl donors for n-glucose to form lactose. The enzyme preparation will not transfer the galactosyl residue...

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Thymidine diphosphate mannose and thymidine diphosphate rhamnose in Streptomyces grieus.

Moflwain, H. (1937). J. chem. Soc. p. 1704. Mahler, H. R., Tomisek, A. & Huennekens, F. M. (1953). Exp. Cell Res. 4, 208. Margoliash, E. (1954). Biochem. J. 56, 529. Meyerhof, 0. (1933). Nature, Lond., 132, 337. Molinari, R. & Lara, F. J. S. (1960). Biochem. J. 75, 57. Nemeth, A. M., Russell, C. S. & Shemin, D. (1957). J. biol. Chem. 229, 415. Neuberg, 0. (1913). Biochem. Z. 49, 502. Nygaard, A...

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Thymidine Diphosphate 4-acetamido-4, 6-dideoxyhexoses. I. Enzymatic Synthesis by Strains of Escherichia Coli.

Several thymidine diphosphate sugar compounds have been isolated from Escherichia coli strains and other microorganisms. TDP-X, isolated from E. coli strain Y-10 by Strominger and Scott (1) and from other strains of E. coli (2) and TDP-X2 isolated from E. coli strain B, by Okazaki, Okazaki, and Kuriki (3) are unusual acetamido sugar-containing nucleotides (4). The sugars are 4-acetamido-4,6-did...

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The enzymatic synthesis of thymidine diphosphate glucose and its conversion to thymidine diphosphate rhamnose.

. Studies from several laboratories have shown that labeled glucose is converted to B-deoxy hexoses without randomization of the label when microorganisms are grown on n-glucose-l-C14 or n-glucose-MY4 as the sole source of carbohydrate (l-3). Evidence of this type has been interpreted to indicate a conversion of the intact hexose moiety to the deoxy compound without scission of the hexose carbo...

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The Nucleotide Specificity and Feedback Control of Thymidine Diphosphate D-glucose Pyrophosphorylase.

A large number of nucleotide diphosphate sugar pyrophosphorylases have been described (for review see References 1 and 2), but only a few of these have been purified to any significant extent. In this paper we report the further purification of deoxythymidine diphosphate n-glucose pyrophosphorylase from Pseudomonas ueruginosa (3). With this partially purified enzyme, it is possible to show that...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1967

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.93.6.2017-2019.1967